Title |
Enantioselective Induction of a Glutathione-S-Transferase, a Glutathione Transporter and an ABC Transporter in Maize by Metolachlor and Its (S)-Isomer
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Published in |
PLOS ONE, October 2012
|
DOI | 10.1371/journal.pone.0048085 |
Pubmed ID | |
Authors |
Sen Pang, Zhaojin Ran, Zhiqian Liu, Xiaoyu Song, Liusheng Duan, Xuefeng Li, Chengju Wang |
Abstract |
The metabolism of chiral herbicides in plants remains poorly understood. Glutathione conjugation reactions are one of the principal mechanisms that plants utilize to detoxify xenobiotics. The induction by rac- and S-metolachlor of the expression of three genes, ZmGST27, ZmGT1 and ZmMRP1, encoding respectively a glutathione-S-transferase, a glutathione transporter and an ATP-binding cassette (ABC) transporter was studied in maize. The results demonstrate that the inducing effect of rac- and S-metolachlor on the expression of ZmGST27 and ZmGT1 is comparable. However, the inducing effect of rac-metolachlor on ZmMRP1 expression is more pronounced than that of S-metolachlor. Furthermore, vanadate, an ABC transporter inhibitor, could greatly reduce the difference in herbicidal activity between rac- and S-metolachlor. These results suggest that the ABC transporters may preferentially transport conjugates of rac-metolachlor, leading to a faster metabolism of the latter. Through comparing the expression of ZmGST27, ZmMRP1 and ZmGT1 after treatment by rac- and S-metolachlor, we provide novel insights into the metabolic processes of chiral herbicides in plants. |
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Geographical breakdown
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Demographic breakdown
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Student > Master | 6 | 21% |
Researcher | 5 | 18% |
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Other | 1 | 4% |
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Chemistry | 1 | 4% |
Engineering | 1 | 4% |
Other | 0 | 0% |
Unknown | 6 | 21% |