Title |
Autophagy Creates a CTL Epitope That Mimics Tumor-Associated Antigens
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Published in |
PLOS ONE, October 2012
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DOI | 10.1371/journal.pone.0047126 |
Pubmed ID | |
Authors |
Ayako Demachi-Okamura, Hiroki Torikai, Yoshiki Akatsuka, Hiroyuki Miyoshi, Tamotsu Yoshimori, Kiyotaka Kuzushima |
Abstract |
The detailed mechanisms responsible for processing tumor-associated antigens and presenting them to CTLs remain to be fully elucidated. In this study, we demonstrate a unique CTL epitope generated from the ubiquitous protein puromycin-sensitive aminopeptidase, which is presented via HLA-A24 on leukemic and pancreatic cancer cells but not on normal fibroblasts or EBV-transformed B lymphoblastoid cells. The generation of this epitope requires proteasomal digestion and transportation from the endoplasmic reticulum to the Golgi apparatus and is sensitive to chloroquine-induced inhibition of acidification inside the endosome/lysosome. Epitope liberation depends on constitutively active autophagy, as confirmed with immunocytochemistry for the autophagosome marker LC3 as well as RNA interference targeting two different autophagy-related genes. Therefore, ubiquitously expressed proteins may be sources of specific tumor-associated antigens when processed through a unique mechanism involving autophagy. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 27% |
Researcher | 5 | 19% |
Student > Bachelor | 4 | 15% |
Student > Doctoral Student | 2 | 8% |
Student > Master | 2 | 8% |
Other | 4 | 15% |
Unknown | 2 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 11 | 42% |
Medicine and Dentistry | 7 | 27% |
Biochemistry, Genetics and Molecular Biology | 4 | 15% |
Immunology and Microbiology | 2 | 8% |
Social Sciences | 1 | 4% |
Other | 0 | 0% |
Unknown | 1 | 4% |