| Title |
A Facile and Specific Assay for Quantifying MicroRNA by an Optimized RT-qPCR Approach
|
|---|---|
| Published in |
PLOS ONE, October 2012
|
| DOI | 10.1371/journal.pone.0046890 |
| Pubmed ID | |
| Authors |
Qian Mei, Xiang Li, Yuanguang Meng, Zhiqiang Wu, Mingzhou Guo, Yali Zhao, Xiaobing Fu, Weidong Han |
| Abstract |
The spatiotemporal expression patterns of microRNAs (miRNAs) are important to the verification of their predicted function. RT-qPCR is the accepted technique for the quantification of miRNA expression; however, stem-loop RT-PCR and poly(T)-adapter assay, the two most frequently used methods, are not very convenient in practice and have poor specificity, respectively. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 1% |
| United States | 1 | 1% |
| Denmark | 1 | 1% |
| Unknown | 88 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 27 | 30% |
| Student > Ph. D. Student | 19 | 21% |
| Student > Master | 11 | 12% |
| Student > Bachelor | 8 | 9% |
| Student > Doctoral Student | 4 | 4% |
| Other | 10 | 11% |
| Unknown | 12 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 31 | 34% |
| Biochemistry, Genetics and Molecular Biology | 26 | 29% |
| Immunology and Microbiology | 4 | 4% |
| Unspecified | 2 | 2% |
| Veterinary Science and Veterinary Medicine | 2 | 2% |
| Other | 9 | 10% |
| Unknown | 17 | 19% |