| Title |
Selection and Application of ssDNA Aptamers to Detect Active TB from Sputum Samples
|
|---|---|
| Published in |
PLOS ONE, October 2012
|
| DOI | 10.1371/journal.pone.0046862 |
| Pubmed ID | |
| Authors |
Lia S. Rotherham, Charlotte Maserumule, Keertan Dheda, Jacques Theron, Makobetsa Khati |
| Abstract |
Despite the enormous global burden of tuberculosis (TB), conventional approaches to diagnosis continue to rely on tests that have major drawbacks. The improvement of TB diagnostics relies, not only on good biomarkers, but also upon accurate detection methodologies. The 10-kDa culture filtrate protein (CFP-10) and the 6-kDa early secreted antigen target (ESAT-6) are potent T-cell antigens that are recognised by over 70% of TB patients. Aptamers, a novel sensitive and specific class of detection molecules, has hitherto, not been raised to these relatively TB-specific antigens. |
Mendeley readers
The data shown below were compiled from readership statistics for 155 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | <1% |
| South Africa | 1 | <1% |
| United Kingdom | 1 | <1% |
| Canada | 1 | <1% |
| Belgium | 1 | <1% |
| China | 1 | <1% |
| Unknown | 149 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 36 | 23% |
| Student > Ph. D. Student | 31 | 20% |
| Student > Master | 17 | 11% |
| Student > Doctoral Student | 15 | 10% |
| Student > Bachelor | 12 | 8% |
| Other | 24 | 15% |
| Unknown | 20 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 34 | 22% |
| Agricultural and Biological Sciences | 33 | 21% |
| Chemistry | 15 | 10% |
| Medicine and Dentistry | 15 | 10% |
| Engineering | 8 | 5% |
| Other | 19 | 12% |
| Unknown | 31 | 20% |