Title |
N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor
|
---|---|
Published in |
PLOS ONE, October 2012
|
DOI | 10.1371/journal.pone.0046039 |
Pubmed ID | |
Authors |
Yaozhong Zou, William I. Weis, Brian K. Kobilka |
Abstract |
A highly crystallizable T4 lysozyme (T4L) was fused to the N-terminus of the β(2) adrenergic receptor (β(2)AR), a G-protein coupled receptor (GPCR) for catecholamines. We demonstrate that the N-terminal fused T4L is sufficiently rigid relative to the receptor to facilitate crystallogenesis without thermostabilizing mutations or the use of a stabilizing antibody, G protein, or protein fused to the 3rd intracellular loop. This approach adds to the protein engineering strategies that enable crystallographic studies of GPCRs alone or in complex with a signaling partner. |
X Demographics
The data shown below were collected from the profiles of 25 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 16% |
United Kingdom | 2 | 8% |
Indonesia | 1 | 4% |
Guatemala | 1 | 4% |
Australia | 1 | 4% |
Peru | 1 | 4% |
France | 1 | 4% |
Chile | 1 | 4% |
Canada | 1 | 4% |
Other | 3 | 12% |
Unknown | 9 | 36% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 18 | 72% |
Scientists | 6 | 24% |
Science communicators (journalists, bloggers, editors) | 1 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 261 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 2% |
United Kingdom | 2 | <1% |
Denmark | 2 | <1% |
Austria | 2 | <1% |
Germany | 1 | <1% |
Switzerland | 1 | <1% |
France | 1 | <1% |
Unknown | 248 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 65 | 25% |
Researcher | 61 | 23% |
Student > Master | 35 | 13% |
Student > Doctoral Student | 20 | 8% |
Student > Bachelor | 19 | 7% |
Other | 37 | 14% |
Unknown | 24 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 96 | 37% |
Biochemistry, Genetics and Molecular Biology | 78 | 30% |
Chemistry | 31 | 12% |
Medicine and Dentistry | 6 | 2% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 2% |
Other | 16 | 6% |
Unknown | 29 | 11% |