Title |
BioFET-SIM Web Interface: Implementation and Two Applications
|
---|---|
Published in |
PLOS ONE, October 2012
|
DOI | 10.1371/journal.pone.0045379 |
Pubmed ID | |
Authors |
Martin R. Hediger, Jan H. Jensen, Luca De Vico |
Abstract |
We present a web interface which allows us to conveniently set up calculations based on the BioFET-SIM model. With the interface, the signal of a BioFET sensor can be calculated depending on its parameters, as well as the signal dependence on pH. As an illustration, two case studies are presented. In the first case, a generic peptide with opposite charges on both ends is inverted in orientation on a semiconducting nanowire surface leading to a corresponding change in sign of the computed sensitivity of the device. In the second case, the binding of an antibody/antigen complex on the nanowire surface is studied in terms of orientation and analyte/nanowire surface distance. We demonstrate how the BioFET-SIM web interface can aid in the understanding of experimental data and postulate alternative ways of antibody/antigen orientation on the nanowire surface. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 58% |
Professor > Associate Professor | 3 | 25% |
Researcher | 1 | 8% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 4 | 33% |
Physics and Astronomy | 3 | 25% |
Immunology and Microbiology | 1 | 8% |
Materials Science | 1 | 8% |
Chemistry | 1 | 8% |
Other | 0 | 0% |
Unknown | 2 | 17% |