Title |
Distinct Licensing of IL-18 and IL-1β Secretion in Response to NLRP3 Inflammasome Activation
|
---|---|
Published in |
PLOS ONE, September 2012
|
DOI | 10.1371/journal.pone.0045186 |
Pubmed ID | |
Authors |
Rebecca L. Schmidt, Laurel L. Lenz |
Abstract |
Inflammasome activation permits processing of interleukins (IL)-1β and 18 and elicits cell death (pyroptosis). Whether these responses are independently licensed or are "hard-wired" consequences of caspase-1 (casp1) activity has not been clear. Here, we show that that each of these responses is independently regulated following activation of NLRP3 inflammasomes by a "non-canonical" stimulus, the secreted Listeria monocytogenes (Lm) p60 protein. Primed murine dendritic cells (DCs) responded to p60 stimulation with reactive oxygen species (ROS) production and secretion of IL-1β and IL-18 but not pyroptosis. Inhibitors of ROS production inhibited secretion of IL-1β, but did not impair IL-18 secretion. Furthermore, DCs from caspase-11 (casp11)-deficient 129S6 mice failed to secrete IL-1β in response to p60 but were fully responsive for IL-18 secretion. These findings reveal that there are distinct licensing requirements for processing of IL-18 versus IL-1β by NLRP3 inflammasomes. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Luxembourg | 2 | 2% |
France | 1 | <1% |
China | 1 | <1% |
United Kingdom | 1 | <1% |
Japan | 1 | <1% |
Spain | 1 | <1% |
Unknown | 95 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 22 | 22% |
Researcher | 21 | 21% |
Student > Master | 16 | 16% |
Student > Bachelor | 6 | 6% |
Professor | 5 | 5% |
Other | 18 | 18% |
Unknown | 14 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 34 | 33% |
Immunology and Microbiology | 19 | 19% |
Medicine and Dentistry | 17 | 17% |
Biochemistry, Genetics and Molecular Biology | 8 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 2% |
Other | 6 | 6% |
Unknown | 16 | 16% |