Title |
Designing and Testing Broadly-Protective Filoviral Vaccines Optimized for Cytotoxic T-Lymphocyte Epitope Coverage
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Published in |
PLOS ONE, October 2012
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DOI | 10.1371/journal.pone.0044769 |
Pubmed ID | |
Authors |
Paul W. Fenimore, Majidat A. Muhammad, William M. Fischer, Brian T. Foley, Russell R. Bakken, James R. Thurmond, Karina Yusim, Hyejin Yoon, Michael Parker, Mary Kate Hart, John M. Dye, Bette Korber, Carla Kuiken |
Abstract |
We report the rational design and in vivo testing of mosaic proteins for a polyvalent pan-filoviral vaccine using a computational strategy designed for the Human Immunodeficiency Virus type 1 (HIV-1) but also appropriate for Hepatitis C virus (HCV) and potentially other diverse viruses. Mosaics are sets of artificial recombinant proteins that are based on natural proteins. The recombinants are computationally selected using a genetic algorithm to optimize the coverage of potential cytotoxic T lymphocyte (CTL) epitopes. Because evolutionary history differs markedly between HIV-1 and filoviruses, we devised an adapted computational technique that is effective for sparsely sampled taxa; our first significant result is that the mosaic technique is effective in creating high-quality mosaic filovirus proteins. The resulting coverage of potential epitopes across filovirus species is superior to coverage by any natural variants, including current vaccine strains with demonstrated cross-reactivity. The mosaic cocktails are also robust: mosaics substantially outperformed natural strains when computationally tested against poorly sampled species and more variable genes. Furthermore, in a computational comparison of cross-reactive potential a design constructed prior to the Bundibugyo outbreak performed nearly as well against all species as an updated design that included Bundibugyo. These points suggest that the mosaic designs would be more resilient than natural-variant vaccines against future Ebola outbreaks dominated by novel viral variants. We demonstrate in vivo immunogenicity and protection against a heterologous challenge in a mouse model. This design work delineates the likely requirements and limitations on broadly-protective filoviral CTL vaccines. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Netherlands | 1 | 2% |
Australia | 1 | 2% |
India | 1 | 2% |
United Kingdom | 1 | 2% |
Canada | 1 | 2% |
Denmark | 1 | 2% |
Unknown | 56 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 14 | 23% |
Researcher | 10 | 16% |
Student > Bachelor | 8 | 13% |
Student > Master | 6 | 10% |
Librarian | 5 | 8% |
Other | 13 | 21% |
Unknown | 6 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 19 | 31% |
Agricultural and Biological Sciences | 11 | 18% |
Biochemistry, Genetics and Molecular Biology | 7 | 11% |
Nursing and Health Professions | 4 | 6% |
Immunology and Microbiology | 3 | 5% |
Other | 8 | 13% |
Unknown | 10 | 16% |