| Title |
Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency
|
|---|---|
| Published in |
PLOS ONE, August 2012
|
| DOI | 10.1371/journal.pone.0043708 |
| Pubmed ID | |
| Authors |
Jayne M. Squirrell, Jimmy J. Fong, Carlos A. Ariza, Amber Mael, Kassondra Meyer, Nirupama K. Shevde, Avtar Roopra, Gary E. Lyons, Timothy J. Kamp, Kevin W. Eliceiri, Brenda M. Ogle |
| Abstract |
The therapeutic potential of stem cells is limited by the non-uniformity of their phenotypic state. Thus it would be advantageous to noninvasively monitor stem cell status. Driven by this challenge, we employed multidimensional multiphoton microscopy to quantify changes in endogenous fluorescence occurring with pluripotent stem cell differentiation. We found that global and cellular-scale fluorescence lifetime of human embryonic stem cells (hESC) and murine embryonic stem cells (mESC) consistently decreased with differentiation. Less consistent were trends in endogenous fluorescence intensity with differentiation, suggesting intensity is more readily impacted by nuances of species and scale of analysis. What emerges is a practical and accessible approach to evaluate, and ultimately enrich, living stem cell populations based on changes in metabolism that could be exploited for both research and clinical applications. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 50% |
| United States | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 9% |
| Portugal | 1 | 2% |
| Unknown | 39 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 27% |
| Researcher | 9 | 20% |
| Professor | 6 | 14% |
| Student > Master | 4 | 9% |
| Other | 3 | 7% |
| Other | 6 | 14% |
| Unknown | 4 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 25% |
| Biochemistry, Genetics and Molecular Biology | 9 | 20% |
| Physics and Astronomy | 4 | 9% |
| Engineering | 4 | 9% |
| Medicine and Dentistry | 3 | 7% |
| Other | 7 | 16% |
| Unknown | 6 | 14% |