| Title |
KDNA Genetic Signatures Obtained by LSSP-PCR Analysis of Leishmania (Leishmania) infantum Isolated from the New and the Old World
|
|---|---|
| Published in |
PLOS ONE, August 2012
|
| DOI | 10.1371/journal.pone.0043363 |
| Pubmed ID | |
| Authors |
Janaína Sousa Campos Alvarenga, Carla Maia Ligeiro, Célia Maria Ferreira Gontijo, Sofia Cortes, Lenea Campino, Annamaria Ravara Vago, Maria Norma Melo |
| Abstract |
Visceral Leishmaniasis (VL) caused by species from the Leishmania donovani complex is the most severe form of the disease, lethal if untreated. VL caused by Leishmania infantum is a zoonosis with an increasing number of human cases and millions of dogs infected in the Old and the New World. In this study, L. infantum (syn. L.chagasi) strains were isolated from human and canine VL cases. The strains were obtained from endemic areas from Brazil and Portugal and their genetic polymorphism was ascertained using the LSSP-PCR (Low-Stringency Single Specific Primer PCR) technique for analyzing the kinetoplastid DNA (kDNA) minicircles hypervariable region. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 2% |
| Unknown | 46 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 15% |
| Student > Master | 7 | 15% |
| Student > Ph. D. Student | 5 | 11% |
| Student > Doctoral Student | 4 | 9% |
| Student > Bachelor | 4 | 9% |
| Other | 8 | 17% |
| Unknown | 12 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 21% |
| Medicine and Dentistry | 7 | 15% |
| Veterinary Science and Veterinary Medicine | 6 | 13% |
| Biochemistry, Genetics and Molecular Biology | 4 | 9% |
| Immunology and Microbiology | 3 | 6% |
| Other | 4 | 9% |
| Unknown | 13 | 28% |