Title |
Simultaneous Detection of Ca2+ and Diacylglycerol Signaling in Living Cells
|
---|---|
Published in |
PLOS ONE, August 2012
|
DOI | 10.1371/journal.pone.0042791 |
Pubmed ID | |
Authors |
Paul Tewson, Mara Westenberg, Yongxin Zhao, Robert E. Campbell, Anne Marie Quinn, Thomas E. Hughes |
Abstract |
Phospholipase C produces two second messengers--diacylglycerol (DAG), which remains in the membrane, and inositol triphosphate (IP(3)), which triggers the release of calcium ions (Ca(2+)) from intracellular stores. Genetically encoded sensors based on a single circularly permuted fluorescent protein (FP) are robust tools for studying intracellular Ca(2+) dynamics. We have developed a robust sensor for DAG based on a circularly permuted green FP that can be co-imaged with the red fluorescent Ca(2+) sensor R-GECO for simultaneous measurement of both second messengers. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 40% |
Unknown | 3 | 60% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 80% |
Scientists | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 129 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 129 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 37 | 29% |
Student > Ph. D. Student | 25 | 19% |
Student > Master | 14 | 11% |
Student > Bachelor | 10 | 8% |
Student > Doctoral Student | 6 | 5% |
Other | 16 | 12% |
Unknown | 21 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 47 | 36% |
Biochemistry, Genetics and Molecular Biology | 28 | 22% |
Medicine and Dentistry | 7 | 5% |
Chemistry | 6 | 5% |
Neuroscience | 6 | 5% |
Other | 13 | 10% |
Unknown | 22 | 17% |