Title |
Evaluating the Ribosomal Internal Transcribed Spacer (ITS) as a Candidate Dinoflagellate Barcode Marker
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Published in |
PLOS ONE, August 2012
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DOI | 10.1371/journal.pone.0042780 |
Pubmed ID | |
Authors |
Rowena F. Stern, Robert A. Andersen, Ian Jameson, Frithjof C. Küpper, Mary-Alice Coffroth, Daniel Vaulot, Florence Le Gall, Benoît Véron, Jerry J. Brand, Hayley Skelton, Fumai Kasai, Emily L. Lilly, Patrick J. Keeling |
Abstract |
DNA barcoding offers an efficient way to determine species identification and to measure biodiversity. For dinoflagellates, an ancient alveolate group of about 2000 described extant species, DNA barcoding studies have revealed large amounts of unrecognized species diversity, most of which is not represented in culture collections. To date, two mitochondrial gene markers, Cytochrome Oxidase I (COI) and Cytochrome b oxidase (COB), have been used to assess DNA barcoding in dinoflagellates, and both failed to amplify all taxa and suffered from low resolution. Nevertheless, both genes yielded many examples of morphospecies showing cryptic speciation and morphologically distinct named species being genetically similar, highlighting the need for a common marker. For example, a large number of cultured Symbiodinium strains have neither taxonomic identification, nor a common measure of diversity that can be used to compare this genus to other dinoflagellates. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Belgium | 1 | 33% |
Canada | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 3 | 2% |
France | 2 | 1% |
Canada | 2 | 1% |
New Zealand | 1 | <1% |
United Kingdom | 1 | <1% |
Japan | 1 | <1% |
Argentina | 1 | <1% |
Unknown | 164 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 41 | 23% |
Researcher | 36 | 21% |
Student > Master | 22 | 13% |
Student > Bachelor | 17 | 10% |
Professor | 9 | 5% |
Other | 26 | 15% |
Unknown | 24 | 14% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 88 | 50% |
Biochemistry, Genetics and Molecular Biology | 22 | 13% |
Environmental Science | 17 | 10% |
Medicine and Dentistry | 4 | 2% |
Earth and Planetary Sciences | 3 | 2% |
Other | 7 | 4% |
Unknown | 34 | 19% |