Title |
Metagenomes of the Picoalga Bathycoccus from the Chile Coastal Upwelling
|
---|---|
Published in |
PLOS ONE, June 2012
|
DOI | 10.1371/journal.pone.0039648 |
Pubmed ID | |
Authors |
Daniel Vaulot, Cécile Lepère, Eve Toulza, Rodrigo De la Iglesia, Julie Poulain, Frédéric Gaboyer, Hervé Moreau, Klaas Vandepoele, Osvaldo Ulloa, Frederick Gavory, Gwenael Piganeau |
Abstract |
Among small photosynthetic eukaryotes that play a key role in oceanic food webs, picoplanktonic Mamiellophyceae such as Bathycoccus, Micromonas, and Ostreococcus are particularly important in coastal regions. By using a combination of cell sorting by flow cytometry, whole genome amplification (WGA), and 454 pyrosequencing, we obtained metagenomic data for two natural picophytoplankton populations from the coastal upwelling waters off central Chile. About 60% of the reads of each sample could be mapped to the genome of Bathycoccus strain from the Mediterranean Sea (RCC1105), representing a total of 9 Mbp (sample T142) and 13 Mbp (sample T149) of non-redundant Bathycoccus genome sequences. WGA did not amplify all regions uniformly, resulting in unequal coverage along a given chromosome and between chromosomes. The identity at the DNA level between the metagenomes and the cultured genome was very high (96.3% identical bases for the three larger chromosomes over a 360 kbp alignment). At least two to three different genotypes seemed to be present in each natural sample based on read mapping to Bathycoccus RCC1105 genome. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 20% |
France | 1 | 20% |
United Kingdom | 1 | 20% |
Egypt | 1 | 20% |
Unknown | 1 | 20% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 3 | 60% |
Members of the public | 2 | 40% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Chile | 5 | 5% |
United States | 2 | 2% |
France | 1 | 1% |
Canada | 1 | 1% |
Sweden | 1 | 1% |
Unknown | 82 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 31 | 34% |
Student > Ph. D. Student | 22 | 24% |
Student > Doctoral Student | 7 | 8% |
Professor | 6 | 7% |
Student > Master | 6 | 7% |
Other | 14 | 15% |
Unknown | 6 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 51 | 55% |
Environmental Science | 14 | 15% |
Biochemistry, Genetics and Molecular Biology | 7 | 8% |
Earth and Planetary Sciences | 5 | 5% |
Immunology and Microbiology | 2 | 2% |
Other | 4 | 4% |
Unknown | 9 | 10% |