| Title |
New Insights into the Mechanisms of Embryonic Stem Cell Self-Renewal under Hypoxia: A Multifactorial Analysis Approach
|
|---|---|
| Published in |
PLOS ONE, June 2012
|
| DOI | 10.1371/journal.pone.0038963 |
| Pubmed ID | |
| Authors |
Hélder S. C. Barbosa, Tiago G. Fernandes, Tiago P. Dias, Maria Margarida Diogo, Joaquim M. S. Cabral |
| Abstract |
Previous reports have shown that culturing mouse embryonic stem (mES) cells at different oxygen tensions originated different cell proliferation patterns and commitment stages depending on which signaling pathways are activated or inhibited to support the pluripotency state. Herein we provide new insights into the mechanisms by which oxygen is influencing mES cell self-renewal and pluripotency. A multifactorial approach was developed to rationally evaluate the singular and interactive control of MEK/ERK pathway, GSK-3 inhibition, and LIF/STAT3 signaling at physiological and non-physiological oxygen tensions. Collectively, our methodology revealed a significant role of GSK-3-mediated signaling towards maintenance of mES cell pluripotency at lower O(2) tensions. Given the central role of this signaling pathway, future studies will need to focus on the downstream mechanisms involved in ES cell self-renewal under such conditions, and ultimately how these findings impact human models of pluripotency. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 25% |
| United States | 1 | 25% |
| Spain | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 3% |
| Portugal | 1 | 2% |
| Canada | 1 | 2% |
| Australia | 1 | 2% |
| Unknown | 59 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 27% |
| Student > Master | 14 | 22% |
| Researcher | 8 | 13% |
| Student > Bachelor | 4 | 6% |
| Student > Doctoral Student | 3 | 5% |
| Other | 9 | 14% |
| Unknown | 9 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 38% |
| Biochemistry, Genetics and Molecular Biology | 11 | 17% |
| Medicine and Dentistry | 6 | 9% |
| Engineering | 5 | 8% |
| Chemical Engineering | 2 | 3% |
| Other | 5 | 8% |
| Unknown | 11 | 17% |