| Title |
Targeting Melanoma Metastasis and Immunosuppression with a New Mode of Melanoma Inhibitory Activity (MIA) Protein Inhibition
|
|---|---|
| Published in |
PLOS ONE, May 2012
|
| DOI | 10.1371/journal.pone.0037941 |
| Pubmed ID | |
| Authors |
Jennifer Schmidt, Alexander Riechers, Raphael Stoll, Thomas Amann, Florian Fink, Thilo Spruss, Wolfram Gronwald, Burkhard König, Claus Hellerbrand, Anja Katrin Bosserhoff |
| Abstract |
Melanoma is the most aggressive form of skin cancer, with fast progression and early dissemination mediated by the melanoma inhibitory activity (MIA) protein. Here, we discovered that dimerization of MIA is required for functional activity through mutagenesis of MIA which showed the correlation between dimerization and functional activity. We subsequently identified the dodecapeptide AR71, which prevents MIA dimerization and thereby acts as a MIA inhibitor. Two-dimensional nuclear magnetic resonance (NMR) spectroscopy demonstrated the binding of AR71 to the MIA dimerization domain, in agreement with in vitro and in vivo data revealing reduced cell migration, reduced formation of metastases and increased immune response after AR71 treatment. We believe AR71 is a lead structure for MIA inhibitors. More generally, inhibiting MIA dimerization is a novel therapeutic concept in melanoma therapy. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 23% |
| Student > Bachelor | 7 | 23% |
| Student > Ph. D. Student | 3 | 10% |
| Student > Doctoral Student | 2 | 6% |
| Professor | 2 | 6% |
| Other | 5 | 16% |
| Unknown | 5 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 9 | 29% |
| Agricultural and Biological Sciences | 7 | 23% |
| Biochemistry, Genetics and Molecular Biology | 4 | 13% |
| Medicine and Dentistry | 3 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 5 | 16% |