Title |
Atomic Force Mechanobiology of Pluripotent Stem Cell-Derived Cardiomyocytes
|
---|---|
Published in |
PLOS ONE, May 2012
|
DOI | 10.1371/journal.pone.0037559 |
Pubmed ID | |
Authors |
Jianwei Liu, Ning Sun, Marc A. Bruce, Joseph C. Wu, Manish J. Butte |
Abstract |
We describe a method using atomic force microscopy (AFM) to quantify the mechanobiological properties of pluripotent, stem cell-derived cardiomyocytes, including contraction force, rate, duration, and cellular elasticity. We measured beats from cardiomyocytes derived from induced pluripotent stem cells of healthy subjects and those with dilated cardiomyopathy, and from embryonic stem cell lines. We found that our AFM method could quantitate beat forces of single cells and clusters of cardiomyocytes. We demonstrate the dose-responsive, inotropic effect of norepinephrine and beta-adrenergic blockade of metoprolol. Cardiomyocytes derived from subjects with dilated cardiomyopathy showed decreased force and decreased cellular elasticity compared to controls. This AFM-based method can serve as a screening tool for the development of cardiac-active pharmacological agents, or as a platform for studying cardiomyocyte biology. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 2% |
France | 1 | <1% |
Ireland | 1 | <1% |
Hungary | 1 | <1% |
United Kingdom | 1 | <1% |
Italy | 1 | <1% |
Russia | 1 | <1% |
China | 1 | <1% |
Unknown | 239 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 71 | 28% |
Researcher | 44 | 18% |
Student > Master | 27 | 11% |
Student > Doctoral Student | 17 | 7% |
Student > Bachelor | 17 | 7% |
Other | 37 | 15% |
Unknown | 38 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 50 | 20% |
Agricultural and Biological Sciences | 47 | 19% |
Biochemistry, Genetics and Molecular Biology | 32 | 13% |
Medicine and Dentistry | 19 | 8% |
Physics and Astronomy | 15 | 6% |
Other | 38 | 15% |
Unknown | 50 | 20% |