| Title |
Small, N-Terminal Tags Activate Parkin E3 Ubiquitin Ligase Activity by Disrupting Its Autoinhibited Conformation
|
|---|---|
| Published in |
PLOS ONE, April 2012
|
| DOI | 10.1371/journal.pone.0034748 |
| Pubmed ID | |
| Authors |
Lynn Burchell, Viduth K. Chaugule, Helen Walden |
| Abstract |
Parkin is an E3 ubiquitin ligase, mutations in which cause Autosomal Recessive Parkinson's Disease. Many studies aimed at understanding Parkin function, regulation and dysfunction are performed using N-terminal epitope tags. We report here that the use of small tags such as FLAG, cMyc and HA, influence the physical stability and activity of Parkin in and out of cells, perturbing the autoinhibited native state of Parkin, resulting in an active-for-autoubiquitination species. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Egypt | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Germany | 1 | 2% |
| Canada | 1 | 2% |
| Unknown | 52 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 25% |
| Researcher | 13 | 24% |
| Student > Master | 7 | 13% |
| Student > Bachelor | 5 | 9% |
| Professor | 2 | 4% |
| Other | 4 | 7% |
| Unknown | 10 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 42% |
| Biochemistry, Genetics and Molecular Biology | 9 | 16% |
| Immunology and Microbiology | 3 | 5% |
| Neuroscience | 3 | 5% |
| Chemistry | 2 | 4% |
| Other | 4 | 7% |
| Unknown | 11 | 20% |