| Title |
Effective DNA/RNA Co-Extraction for Analysis of MicroRNAs, mRNAs, and Genomic DNA from Formalin-Fixed Paraffin-Embedded Specimens
|
|---|---|
| Published in |
PLOS ONE, April 2012
|
| DOI | 10.1371/journal.pone.0034683 |
| Pubmed ID | |
| Authors |
Adam Kotorashvili, Andrew Ramnauth, Christina Liu, Juan Lin, Kenny Ye, Ryung Kim, Rachel Hazan, Thomas Rohan, Susan Fineberg, Olivier Loudig |
| Abstract |
Retrospective studies of archived human specimens, with known clinical follow-up, are used to identify predictive and prognostic molecular markers of disease. Due to biochemical differences, however, formalin-fixed paraffin-embedded (FFPE) DNA and RNA have generally been extracted separately from either different tissue sections or from the same section by dividing the digested tissue. The former limits accurate correlation whilst the latter is impractical when utilizing rare or limited archived specimens. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 198 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 1% |
| Brazil | 2 | 1% |
| United Kingdom | 2 | 1% |
| Malaysia | 1 | <1% |
| South Africa | 1 | <1% |
| Germany | 1 | <1% |
| India | 1 | <1% |
| Spain | 1 | <1% |
| Denmark | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 186 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 49 | 25% |
| Student > Ph. D. Student | 38 | 19% |
| Student > Master | 22 | 11% |
| Student > Bachelor | 16 | 8% |
| Student > Doctoral Student | 10 | 5% |
| Other | 37 | 19% |
| Unknown | 26 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 78 | 39% |
| Biochemistry, Genetics and Molecular Biology | 38 | 19% |
| Medicine and Dentistry | 28 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 2% |
| Immunology and Microbiology | 3 | 2% |
| Other | 18 | 9% |
| Unknown | 29 | 15% |