| Title |
HIV-1 Nef Enhances Dendritic Cell-Mediated Viral Transmission to CD4+ T Cells and Promotes T-Cell Activation
|
|---|---|
| Published in |
PLOS ONE, March 2012
|
| DOI | 10.1371/journal.pone.0034521 |
| Pubmed ID | |
| Authors |
Corine St. Gelais, Christopher M. Coleman, Jian-Hua Wang, Li Wu |
| Abstract |
HIV-1 Nef enhances dendritic cell (DC)-mediated viral transmission to CD4(+) T cells, but the underlying mechanism is not fully understood. It is also unknown whether HIV-1 infected DCs play a role in activating CD4(+) T cells and enhancing DC-mediated viral transmission. Here we investigated the role of HIV-1 Nef in DC-mediated viral transmission and HIV-1 infection of primary CD4(+) T cells using wild-type HIV-1 and Nef-mutated viruses. We show that HIV-1 Nef facilitated DC-mediated viral transmission to activated CD4(+) T cells. HIV-1 expressing wild-type Nef enhanced the activation and proliferation of primary resting CD4(+) T cells. However, when co-cultured with HIV-1-infected autologous DCs, there was no significant trend for infection- or Nef-dependent proliferation of resting CD4(+) T cells. Our results suggest an important role of Nef in DC-mediated transmission of HIV-1 to activated CD4(+) T cells and in the activation and proliferation of resting CD4(+) T cells, which likely contribute to viral pathogenesis. |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| South Africa | 1 | 3% |
| Unknown | 29 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 30% |
| Student > Master | 4 | 13% |
| Professor | 3 | 10% |
| Student > Ph. D. Student | 3 | 10% |
| Student > Bachelor | 2 | 7% |
| Other | 6 | 20% |
| Unknown | 3 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 47% |
| Medicine and Dentistry | 5 | 17% |
| Immunology and Microbiology | 5 | 17% |
| Biochemistry, Genetics and Molecular Biology | 2 | 7% |
| Business, Management and Accounting | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 3 | 10% |