Title |
B Cell: T Cell Interactions Occur within Hepatic Granulomas during Experimental Visceral Leishmaniasis
|
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Published in |
PLOS ONE, March 2012
|
DOI | 10.1371/journal.pone.0034143 |
Pubmed ID | |
Authors |
John W. J. Moore, Lynette Beattie, Jane E. Dalton, Benjamin M. J. Owens, Asher Maroof, Mark C. Coles, Paul M. Kaye |
Abstract |
Hepatic resistance to Leishmania donovani infection in mice is associated with the development of granulomas, in which a variety of lymphoid and non-lymphoid populations accumulate. Although previous studies have identified B cells in hepatic granulomas and functional studies in B cell-deficient mice have suggested a role for B cells in the control of experimental visceral leishmaniasis, little is known about the behaviour of B cells in the granuloma microenvironment. Here, we first compared the hepatic B cell population in infected mice, where ≈60% of B cells are located within granulomas, with that of naïve mice. In infected mice, there was a small increase in mIgM(lo)mIgD(+) mature B2 cells, but no enrichment of B cells with regulatory phenotype or function compared to the naïve hepatic B cell population, as assessed by CD1d and CD5 expression and by IL-10 production. Using 2-photon microscopy to quantify the entire intra-granuloma B cell population, in conjunction with the adoptive transfer of polyclonal and HEL-specific BCR-transgenic B cells isolated from L. donovani-infected mice, we demonstrated that B cells accumulate in granulomas over time in an antigen-independent manner. Intra-vital dynamic imaging was used to demonstrate that within the polyclonal B cell population obtained from L. donovani-infected mice, the frequency of B cells that made multiple long contacts with endogenous T cells was greater than that observed using HEL-specific B cells obtained from the same inflammatory environment. These data indicate, therefore, that a subset of this polyclonal B cell population is capable of making cognate interactions with T cells within this unique environment, and provide the first insights into the dynamics of B cells within an inflammatory site. |
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Geographical breakdown
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Demographic breakdown
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Student > Ph. D. Student | 6 | 13% |
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Student > Doctoral Student | 4 | 8% |
Other | 8 | 17% |
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