| Title |
De-Novo Assembly and Analysis of the Heterozygous Triploid Genome of the Wine Spoilage Yeast Dekkera bruxellensis AWRI1499
|
|---|---|
| Published in |
PLOS ONE, March 2012
|
| DOI | 10.1371/journal.pone.0033840 |
| Pubmed ID | |
| Authors |
Chris D. Curtin, Anthony R. Borneman, Paul J. Chambers, Isak S. Pretorius |
| Abstract |
Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 75% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 154 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 3 | 2% |
| France | 2 | 1% |
| Norway | 1 | <1% |
| Netherlands | 1 | <1% |
| Australia | 1 | <1% |
| Uruguay | 1 | <1% |
| Egypt | 1 | <1% |
| Poland | 1 | <1% |
| Unknown | 143 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 34 | 22% |
| Researcher | 29 | 19% |
| Student > Master | 23 | 15% |
| Student > Bachelor | 14 | 9% |
| Professor | 10 | 6% |
| Other | 23 | 15% |
| Unknown | 21 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 85 | 55% |
| Biochemistry, Genetics and Molecular Biology | 25 | 16% |
| Immunology and Microbiology | 4 | 3% |
| Chemistry | 3 | 2% |
| Environmental Science | 2 | 1% |
| Other | 6 | 4% |
| Unknown | 29 | 19% |