Title |
Systemic and Mucosal Immune Responses to Sublingual or Intramuscular Human Papilloma Virus Antigens in Healthy Female Volunteers
|
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Published in |
PLOS ONE, March 2012
|
DOI | 10.1371/journal.pone.0033736 |
Pubmed ID | |
Authors |
Zhiming Huo, Sara L. Bissett, Raphaela Giemza, Simon Beddows, Clarissa Oeser, David J. M. Lewis |
Abstract |
The sublingual route has been proposed as a needle-free option to induce systemic and mucosal immune protection against viral infections. In a translational study of systemic and mucosal humoral immune responses to sublingual or systemically administered viral antigens, eighteen healthy female volunteers aged 19-31 years received three immunizations with a quadravalent Human Papilloma Virus vaccine at 0, 4 and 16 weeks as sublingual drops (SL, n = 12) or intramuscular injection (IM, n = 6). IM antigen delivery induced or boosted HPV-specific serum IgG and pseudovirus-neutralizing antibodies, HPV-specific cervical and vaginal IgG, and elicited circulating IgG and IgA antibody secreting cells. SL antigens induced ~38-fold lower serum and ~2-fold lower cervical/vaginal IgG than IM delivery, and induced or boosted serum virus neutralizing antibody in only 3/12 subjects. Neither route reproducibly induced HPV-specific mucosal IgA. Alternative delivery systems and adjuvants will be required to enhance and evaluate immune responses following sublingual immunization in humans. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Ireland | 1 | 3% |
South Africa | 1 | 3% |
Mexico | 1 | 3% |
Argentina | 1 | 3% |
United States | 1 | 3% |
Unknown | 35 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 23% |
Researcher | 5 | 13% |
Student > Doctoral Student | 4 | 10% |
Other | 4 | 10% |
Student > Master | 4 | 10% |
Other | 7 | 18% |
Unknown | 7 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 25% |
Medicine and Dentistry | 9 | 23% |
Immunology and Microbiology | 4 | 10% |
Engineering | 3 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
Other | 2 | 5% |
Unknown | 10 | 25% |