Title |
Both TLR2 and TRIF Contribute to Interferon-β Production during Listeria Infection
|
---|---|
Published in |
PLOS ONE, March 2012
|
DOI | 10.1371/journal.pone.0033299 |
Pubmed ID | |
Authors |
Camille Aubry, Sinéad C. Corr, Sebastian Wienerroither, Céline Goulard, Ruth Jones, Amanda M. Jamieson, Thomas Decker, Luke A. J. O'Neill, Olivier Dussurget, Pascale Cossart |
Abstract |
Synthesis of interferon-β (IFN-β) is an innate response to cytoplasmic infection with bacterial pathogens. Our recent studies showed that Listeria monocytogenes limits immune detection and IFN-β synthesis via deacetylation of its peptidoglycan, which renders the bacterium resistant to lysozyme degradation. Here, we examined signaling requirements for the massive IFN-β production resulting from the infection of murine macrophages with a mutant strain of L. monocytogenes, ΔpgdA, which is unable to modify its peptidoglycan. We report the identification of unconventional signaling pathways to the IFN-β gene, requiring TLR2 and bacterial internalization. Induction of IFN-β was independent of the Mal/TIRAP adaptor protein but required TRIF and the transcription factors IRF3 and IRF7. These pathways were stimulated to a lesser degree by wild-type L. monocytogenes. They operated in both resident and inflammatory macrophages derived from the peritoneal cavity, but not in bone marrow-derived macrophages. The novelty of our findings thus lies in the first description of TLR2 and TRIF as two critical components leading to the induction of the IFN-β gene and in uncovering that individual macrophage populations adopt different strategies to link pathogen recognition signals to IFN-β gene expression. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 1% |
France | 1 | 1% |
Norway | 1 | 1% |
Unknown | 71 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 21 | 28% |
Researcher | 8 | 11% |
Student > Master | 7 | 9% |
Student > Doctoral Student | 6 | 8% |
Professor | 5 | 7% |
Other | 19 | 26% |
Unknown | 8 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 36 | 49% |
Immunology and Microbiology | 13 | 18% |
Medicine and Dentistry | 5 | 7% |
Biochemistry, Genetics and Molecular Biology | 4 | 5% |
Veterinary Science and Veterinary Medicine | 2 | 3% |
Other | 4 | 5% |
Unknown | 10 | 14% |