Title |
Bleomycin Sensitivity in Escherichia coli is Medium-Dependent
|
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Published in |
PLOS ONE, March 2012
|
DOI | 10.1371/journal.pone.0033256 |
Pubmed ID | |
Authors |
Tao Xu, William Brown, Martin G. Marinus |
Abstract |
Bleomycin (BLM) is a glycopeptide antibiotic and anti-tumor agent that targets primarily the furanose rings of DNA and in the presence of ferrous ions produces oxidative damage and DNA strand breaks. Escherichia coli cells growing in broth medium and exposed to low concentrations of BLM contain double-strand breaks and require homologous recombination to survive. To a lesser extent, the cells also require the abasic (AP) endonucleases associated with base excision repair, presumably to repair oxidative damage. As expected, there is strong induction of the SOS system in treated cells. In contrast, E. coli cells growing in glucose or glycerol minimal medium are resistant to the lethal action of BLM and do not require either homologous recombination functions or AP-endonucleases for survival. DNA ligase activity, however, is needed for cells growing in minimal medium to resist the lethal effects of BLM. There is weak SOS induction in such treated cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 25% |
Student > Bachelor | 4 | 17% |
Student > Master | 3 | 13% |
Student > Ph. D. Student | 2 | 8% |
Other | 2 | 8% |
Other | 2 | 8% |
Unknown | 5 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 29% |
Biochemistry, Genetics and Molecular Biology | 6 | 25% |
Immunology and Microbiology | 2 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Chemistry | 1 | 4% |
Other | 1 | 4% |
Unknown | 6 | 25% |