| Title |
Establishment of an ES Cell-Derived Murine Megakaryocytic Cell Line, MKD1, with Features of Primary Megakaryocyte Progenitors
|
|---|---|
| Published in |
PLOS ONE, March 2012
|
| DOI | 10.1371/journal.pone.0032981 |
| Pubmed ID | |
| Authors |
Hedia Chagraoui, Catherine Porcher |
| Abstract |
Because of the scarcity of megakaryocytes in hematopoietic tissues, studying megakaryopoiesis heavily relies on the availability of appropriate cellular models. Here, we report the establishment of a new mouse embryonic stem (ES) cell-derived megakaryocytic cell line, MKD1. The cells are factor-dependent, their cell surface immunophenotype and gene expression profile closely resemble that of primary megakaryocyte progenitors (MkPs) and they further differentiate along the megakaryocyte lineage upon valproic acid treatment. At a functional level, we show that ablation of SCL expression, a transcription factor critical for MkP maturation, leads to gene expression alterations similar to that observed in primary, Scl-excised MkPs. Moreover, the cell line is amenable to biochemical and transcriptional analyses, as we report for GpVI, a direct target of SCL. Thus, the MKD1 cell line offers a pertinent experimental model to study the cellular and molecular mechanisms underlying MkP biology and more broadly megakaryopoiesis. |
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| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 5% |
| Spain | 1 | 5% |
| United States | 1 | 5% |
| Unknown | 18 | 86% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 33% |
| Student > Ph. D. Student | 5 | 24% |
| Student > Bachelor | 2 | 10% |
| Student > Master | 2 | 10% |
| Lecturer > Senior Lecturer | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 2 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 57% |
| Biochemistry, Genetics and Molecular Biology | 5 | 24% |
| Business, Management and Accounting | 1 | 5% |
| Unknown | 3 | 14% |