Title |
CRIM1 Complexes with ß-catenin and Cadherins, Stabilizes Cell-Cell Junctions and Is Critical for Neural Morphogenesis
|
---|---|
Published in |
PLOS ONE, March 2012
|
DOI | 10.1371/journal.pone.0032635 |
Pubmed ID | |
Authors |
Virgilio G. Ponferrada, Jieqing Fan, Jefferson E. Vallance, Shengyong Hu, Aygun Mamedova, Scott A. Rankin, Matthew Kofron, Aaron M. Zorn, Rashmi S. Hegde, Richard A. Lang |
Abstract |
In multicellular organisms, morphogenesis is a highly coordinated process that requires dynamically regulated adhesion between cells. An excellent example of cellular morphogenesis is the formation of the neural tube from the flattened epithelium of the neural plate. Cysteine-rich motor neuron protein 1 (CRIM1) is a single-pass (type 1) transmembrane protein that is expressed in neural structures beginning at the neural plate stage. In the frog Xenopus laevis, loss of function studies using CRIM1 antisense morpholino oligonucleotides resulted in a failure of neural development. The CRIM1 knockdown phenotype was, in some cases, mild and resulted in perturbed neural fold morphogenesis. In severely affected embryos there was a dramatic failure of cell adhesion in the neural plate and complete absence of neural structures subsequently. Investigation of the mechanism of CRIM1 function revealed that it can form complexes with ß-catenin and cadherins, albeit indirectly, via the cytosolic domain. Consistent with this, CRIM1 knockdown resulted in diminished levels of cadherins and ß-catenin in junctional complexes in the neural plate. We conclude that CRIM1 is critical for cell-cell adhesion during neural development because it is required for the function of cadherin-dependent junctions. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 8% |
Japan | 1 | 4% |
United Kingdom | 1 | 4% |
Unknown | 22 | 85% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 31% |
Student > Ph. D. Student | 6 | 23% |
Professor | 3 | 12% |
Student > Doctoral Student | 2 | 8% |
Student > Bachelor | 2 | 8% |
Other | 5 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 15 | 58% |
Biochemistry, Genetics and Molecular Biology | 3 | 12% |
Chemistry | 2 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Computer Science | 1 | 4% |
Other | 3 | 12% |
Unknown | 1 | 4% |