Title |
Isothermal Amplification Using a Chemical Heating Device for Point-of-Care Detection of HIV-1
|
---|---|
Published in |
PLOS ONE, February 2012
|
DOI | 10.1371/journal.pone.0031432 |
Pubmed ID | |
Authors |
Kelly A. Curtis, Donna L. Rudolph, Irene Nejad, Jered Singleton, Andy Beddoe, Bernhard Weigl, Paul LaBarre, S. Michele Owen |
Abstract |
To date, the use of traditional nucleic acid amplification tests (NAAT) for detection of HIV-1 DNA or RNA has been restricted to laboratory settings due to time, equipment, and technical expertise requirements. The availability of a rapid NAAT with applicability for resource-limited or point-of-care (POC) settings would fill a great need in HIV diagnostics, allowing for timely diagnosis or confirmation of infection status, as well as facilitating the diagnosis of acute infection, screening and evaluation of infants born to HIV-infected mothers. Isothermal amplification methods, such as reverse-transcription, loop-mediated isothermal amplification (RT-LAMP), exhibit characteristics that are ideal for POC settings, since they are typically quicker, easier to perform, and allow for integration into low-tech, portable heating devices. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 1% |
Germany | 1 | <1% |
Malaysia | 1 | <1% |
South Africa | 1 | <1% |
Brazil | 1 | <1% |
Canada | 1 | <1% |
Madagascar | 1 | <1% |
Unknown | 193 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 54 | 27% |
Student > Ph. D. Student | 44 | 22% |
Student > Master | 15 | 7% |
Student > Bachelor | 15 | 7% |
Student > Postgraduate | 10 | 5% |
Other | 29 | 14% |
Unknown | 35 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 46 | 23% |
Engineering | 38 | 19% |
Biochemistry, Genetics and Molecular Biology | 24 | 12% |
Medicine and Dentistry | 16 | 8% |
Chemistry | 6 | 3% |
Other | 26 | 13% |
Unknown | 46 | 23% |