| Title |
Automated Workflow for Preparation of cDNA for Cap Analysis of Gene Expression on a Single Molecule Sequencer
|
|---|---|
| Published in |
PLOS ONE, January 2012
|
| DOI | 10.1371/journal.pone.0030809 |
| Pubmed ID | |
| Authors |
Masayoshi Itoh, Miki Kojima, Sayaka Nagao-Sato, Eri Saijo, Timo Lassmann, Mutsumi Kanamori-Katayama, Ai Kaiho, Marina Lizio, Hideya Kawaji, Piero Carninci, Alistair R. R. Forrest, Yoshihide Hayashizaki |
| Abstract |
Cap analysis of gene expression (CAGE) is a 5' sequence tag technology to globally determine transcriptional starting sites in the genome and their expression levels and has most recently been adapted to the HeliScope single molecule sequencer. Despite significant simplifications in the CAGE protocol, it has until now been a labour intensive protocol. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 2 | 3% |
| United Kingdom | 1 | 1% |
| Sweden | 1 | 1% |
| Unknown | 63 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 24% |
| Student > Ph. D. Student | 12 | 18% |
| Student > Master | 7 | 10% |
| Professor > Associate Professor | 6 | 9% |
| Other | 5 | 7% |
| Other | 15 | 22% |
| Unknown | 6 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 36 | 54% |
| Biochemistry, Genetics and Molecular Biology | 10 | 15% |
| Medicine and Dentistry | 5 | 7% |
| Engineering | 3 | 4% |
| Chemistry | 2 | 3% |
| Other | 4 | 6% |
| Unknown | 7 | 10% |