Title |
Tyrosine Phosphorylation of Rac1: A Role in Regulation of Cell Spreading
|
---|---|
Published in |
PLOS ONE, December 2011
|
DOI | 10.1371/journal.pone.0028587 |
Pubmed ID | |
Authors |
Fumin Chang, Christopher Lemmon, Daniel Lietha, Michael Eck, Lewis Romer |
Abstract |
Rac1 influences a multiplicity of vital cellular- and tissue-level control functions, making it an important candidate for targeted therapeutics. The activity of the Rho family member Cdc42 has been shown to be modulated by tyrosine phosphorylation at position 64. We therefore investigated consequences of the point mutations Y64F and Y64D in Rac1. Both mutations altered cell spreading from baseline in the settings of wild type, constitutively active, or dominant negative Rac1 expression, and were accompanied by differences in Rac1 targeting to focal adhesions. Rac1-Y64F displayed increased GTP-binding, increased association with βPIX, and reduced binding with RhoGDI as compared with wild type Rac1. Rac1-Y64D had less binding to PAK than Rac1-WT or Rac1-64F. In vitro assays demonstrated that Y64 in Rac1 is a target for FAK and Src. Taken together, these data suggest a mechanism for the regulation of Rac1 activity by non-receptor tyrosine kinases, with consequences for membrane extension. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 2% |
Unknown | 84 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 25 | 29% |
Researcher | 13 | 15% |
Student > Bachelor | 11 | 13% |
Student > Master | 10 | 12% |
Student > Doctoral Student | 9 | 10% |
Other | 9 | 10% |
Unknown | 9 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 31 | 36% |
Biochemistry, Genetics and Molecular Biology | 30 | 35% |
Medicine and Dentistry | 4 | 5% |
Neuroscience | 3 | 3% |
Engineering | 3 | 3% |
Other | 5 | 6% |
Unknown | 10 | 12% |