| Title |
Dengue Reporter Virus Particles for Measuring Neutralizing Antibodies against Each of the Four Dengue Serotypes
|
|---|---|
| Published in |
PLOS ONE, November 2011
|
| DOI | 10.1371/journal.pone.0027252 |
| Pubmed ID | |
| Authors |
Kimberly Mattia, Bridget A. Puffer, Katherine L. Williams, Ritela Gonzalez, Meredith Murray, Emily Sluzas, Dan Pagano, Sandya Ajith, Megan Bower, Eli Berdougo, Eva Harris, Benjamin J. Doranz |
| Abstract |
The lack of reliable, high-throughput tools for characterizing anti-dengue virus (DENV) antibodies in large numbers of serum samples has been an obstacle in understanding the impact of neutralizing antibodies on disease progression and vaccine efficacy. A reporter system using pseudoinfectious DENV reporter virus particles (RVPs) was previously developed by others to facilitate the genetic manipulation and biological characterization of DENV virions. In the current study, we demonstrate the diagnostic utility of DENV RVPs for measuring neutralizing antibodies in human serum samples against all four DENV serotypes, with attention to the suitability of DENV RVPs for large-scale, long-term studies. DENV RVPs used against human sera yielded serotype-specific responses and reproducible neutralization titers that were in statistical agreement with Plaque Reduction Neutralization Test (PRNT) results. DENV RVPs were also used to measure neutralization titers against the four DENV serotypes in a panel of human sera from a clinical study of dengue patients. The high-throughput capability, stability, rapidity, and reproducibility of assays using DENV RVPs offer advantages for detecting immune responses that can be applied to large-scale clinical studies of DENV infection and vaccination. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 123 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 2% |
| Germany | 1 | <1% |
| Indonesia | 1 | <1% |
| France | 1 | <1% |
| French Polynesia | 1 | <1% |
| United Kingdom | 1 | <1% |
| Brazil | 1 | <1% |
| Mexico | 1 | <1% |
| Philippines | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 112 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 34 | 28% |
| Student > Ph. D. Student | 31 | 25% |
| Student > Master | 13 | 11% |
| Other | 8 | 7% |
| Student > Bachelor | 7 | 6% |
| Other | 13 | 11% |
| Unknown | 17 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 49 | 40% |
| Immunology and Microbiology | 15 | 12% |
| Biochemistry, Genetics and Molecular Biology | 15 | 12% |
| Medicine and Dentistry | 15 | 12% |
| Veterinary Science and Veterinary Medicine | 2 | 2% |
| Other | 7 | 6% |
| Unknown | 20 | 16% |