Title |
Vaccination of Lactating Dairy Cows for the Prevention of Aflatoxin B1 Carry Over in Milk
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Published in |
PLOS ONE, October 2011
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DOI | 10.1371/journal.pone.0026777 |
Pubmed ID | |
Authors |
Luciano Polonelli, Laura Giovati, Walter Magliani, Stefania Conti, Stefano Sforza, Alessandro Calabretta, Claudio Casoli, Paola Ronzi, Ester Grilli, Antonio Gallo, Francesco Masoero, Gianfranco Piva |
Abstract |
The potential of anaflatoxin B(1) (AnAFB(1)) conjugated to keyhole limpet hemocyanin (KLH) as a vaccine (AnAFB(1)-KLH) in controlling the carry over of the aflatoxin B(1) (AFB(1)) metabolite aflatoxin M(1) (AFM(1)) in cow milk is reported. AFB(1) is the most carcinogenic compound in food and foodstuffs amongst aflatoxins (AFs). AnAFB(1) is AFB(1) chemically modified as AFB(1)-1(O-carboxymethyl) oxime. In comparison to AFB(1), AnAFB(1) has proven to be non-toxic in vitro to human hepatocarcinoma cells and non mutagenic to Salmonella typhimurium strains. AnAFB(1)-KLH was used for immunization of cows proving to induce a long lasting titer of anti-AFB(1) IgG antibodies (Abs) which were cross reactive with AFB(1), AFG(1), and AFG(2). The elicited anti-AFB(1) Abs were able to hinder the secretion of AFM(1) into the milk of cows continuously fed with AFB(1). Vaccination of lactating animals with conjugated AnAFB(1) may represent a solution to the public hazard constituted by milk and cheese contaminated with AFs. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Kenya | 1 | 2% |
Italy | 1 | 2% |
Unknown | 59 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 18 | 30% |
Student > Ph. D. Student | 9 | 15% |
Other | 7 | 11% |
Professor > Associate Professor | 4 | 7% |
Student > Master | 4 | 7% |
Other | 11 | 18% |
Unknown | 8 | 13% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 20 | 33% |
Medicine and Dentistry | 7 | 11% |
Chemistry | 7 | 11% |
Veterinary Science and Veterinary Medicine | 4 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
Other | 11 | 18% |
Unknown | 10 | 16% |