| Title |
A Comparison of Parallel Pyrosequencing and Sanger Clone-Based Sequencing and Its Impact on the Characterization of the Genetic Diversity of HIV-1
|
|---|---|
| Published in |
PLOS ONE, October 2011
|
| DOI | 10.1371/journal.pone.0026745 |
| Pubmed ID | |
| Authors |
Binhua Liang, Ma Luo, Joel Scott-Herridge, Christina Semeniuk, Mark Mendoza, Rupert Capina, Brent Sheardown, Hezhao Ji, Joshua Kimani, Blake T. Ball, Gary Van Domselaar, Morag Graham, Shane Tyler, Steven J. M. Jones, Francis A. Plummer |
| Abstract |
Pyrosequencing technology has the potential to rapidly sequence HIV-1 viral quasispecies without requiring the traditional approach of cloning. In this study, we investigated the utility of ultra-deep pyrosequencing to characterize genetic diversity of the HIV-1 gag quasispecies and assessed the possible contribution of pyrosequencing technology in studying HIV-1 biology and evolution. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 1% |
| France | 1 | 1% |
| Brazil | 1 | 1% |
| Sweden | 1 | 1% |
| United Kingdom | 1 | 1% |
| Canada | 1 | 1% |
| United States | 1 | 1% |
| Unknown | 80 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 27 | 31% |
| Researcher | 18 | 21% |
| Student > Master | 8 | 9% |
| Student > Bachelor | 6 | 7% |
| Student > Doctoral Student | 4 | 5% |
| Other | 13 | 15% |
| Unknown | 11 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 43 | 49% |
| Medicine and Dentistry | 13 | 15% |
| Biochemistry, Genetics and Molecular Biology | 7 | 8% |
| Social Sciences | 4 | 5% |
| Environmental Science | 2 | 2% |
| Other | 3 | 3% |
| Unknown | 15 | 17% |