Title |
Silencing of Aphid Genes by dsRNA Feeding from Plants
|
---|---|
Published in |
PLOS ONE, October 2011
|
DOI | 10.1371/journal.pone.0025709 |
Pubmed ID | |
Authors |
Marco Pitino, Alexander D. Coleman, Massimo E. Maffei, Christopher J. Ridout, Saskia A. Hogenhout |
Abstract |
RNA interference (RNAi) is a valuable reverse genetics tool to study gene function in various organisms, including hemipteran insects such as aphids. Previous work has shown that RNAi-mediated knockdown of pea aphid (Acyrthosiphon pisum) genes can be achieved through direct injection of double-stranded RNA (dsRNA) or small-interfering RNAs (siRNA) into the pea aphid hemolymph or by feeding these insects on artificial diets containing the small RNAs. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
Curaçao | 1 | 9% |
United States | 1 | 9% |
Germany | 1 | 9% |
Japan | 1 | 9% |
Unknown | 7 | 64% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 4 | 36% |
Members of the public | 4 | 36% |
Science communicators (journalists, bloggers, editors) | 2 | 18% |
Practitioners (doctors, other healthcare professionals) | 1 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 305 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | <1% |
France | 2 | <1% |
Nigeria | 2 | <1% |
India | 1 | <1% |
United Kingdom | 1 | <1% |
Kenya | 1 | <1% |
Uganda | 1 | <1% |
Slovenia | 1 | <1% |
Spain | 1 | <1% |
Other | 1 | <1% |
Unknown | 291 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 86 | 28% |
Researcher | 54 | 18% |
Student > Master | 35 | 11% |
Student > Bachelor | 31 | 10% |
Student > Doctoral Student | 21 | 7% |
Other | 38 | 12% |
Unknown | 40 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 202 | 66% |
Biochemistry, Genetics and Molecular Biology | 47 | 15% |
Environmental Science | 3 | <1% |
Immunology and Microbiology | 2 | <1% |
Nursing and Health Professions | 1 | <1% |
Other | 3 | <1% |
Unknown | 47 | 15% |