Title |
Adaptive Evolution of Staphylococcus aureus during Chronic Endobronchial Infection of a Cystic Fibrosis Patient
|
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Published in |
PLOS ONE, September 2011
|
DOI | 10.1371/journal.pone.0024301 |
Pubmed ID | |
Authors |
Paul R. McAdam, Anne Holmes, Kate E. Templeton, J. Ross Fitzgerald |
Abstract |
The molecular adaptation of Staphylococcus aureus to its host during chronic infection is not well understood. Comparative genome sequencing of 3 S. aureus isolates obtained sequentially over 26 months from the airways of a cystic fibrosis patient, revealed variation in phage content, and genetic polymorphisms in genes which influence antibiotic resistance, and global regulation of virulence. The majority of polymorphisms were isolate-specific suggesting the existence of an heterogeneous infecting population that evolved from a single infecting strain of S. aureus. The genetic variation identified correlated with differences in growth rate, hemolytic activity, and antibiotic sensitivity, implying a profound effect on the ecology of S. aureus. In particular, a high frequency of mutations in loci associated with the alternate transcription factor SigB, were observed. The identification of genes under diversifying selection during long-term infection may inform the design of novel therapeutics for the control of refractory chronic infections. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 2 | 2% |
Denmark | 2 | 2% |
Germany | 1 | <1% |
Israel | 1 | <1% |
Netherlands | 1 | <1% |
Sweden | 1 | <1% |
United States | 1 | <1% |
Unknown | 117 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 27 | 21% |
Researcher | 25 | 20% |
Student > Master | 13 | 10% |
Student > Bachelor | 12 | 10% |
Student > Doctoral Student | 7 | 6% |
Other | 25 | 20% |
Unknown | 17 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 51 | 40% |
Immunology and Microbiology | 17 | 13% |
Medicine and Dentistry | 15 | 12% |
Biochemistry, Genetics and Molecular Biology | 13 | 10% |
Veterinary Science and Veterinary Medicine | 3 | 2% |
Other | 3 | 2% |
Unknown | 24 | 19% |