Title |
Genome-Wide Functional Profiling Reveals Genes Required for Tolerance to Benzene Metabolites in Yeast
|
---|---|
Published in |
PLOS ONE, August 2011
|
DOI | 10.1371/journal.pone.0024205 |
Pubmed ID | |
Authors |
Matthew North, Vickram J. Tandon, Reuben Thomas, Alex Loguinov, Inna Gerlovina, Alan E. Hubbard, Luoping Zhang, Martyn T. Smith, Chris D. Vulpe |
Abstract |
Benzene is a ubiquitous environmental contaminant and is widely used in industry. Exposure to benzene causes a number of serious health problems, including blood disorders and leukemia. Benzene undergoes complex metabolism in humans, making mechanistic determination of benzene toxicity difficult. We used a functional genomics approach to identify the genes that modulate the cellular toxicity of three of the phenolic metabolites of benzene, hydroquinone (HQ), catechol (CAT) and 1,2,4-benzenetriol (BT), in the model eukaryote Saccharomyces cerevisiae. Benzene metabolites generate oxidative and cytoskeletal stress, and tolerance requires correct regulation of iron homeostasis and the vacuolar ATPase. We have identified a conserved bZIP transcription factor, Yap3p, as important for a HQ-specific response pathway, as well as two genes that encode putative NAD(P)H:quinone oxidoreductases, PST2 and YCP4. Many of the yeast genes identified have human orthologs that may modulate human benzene toxicity in a similar manner and could play a role in benzene exposure-related disease. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 2% |
Unknown | 63 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 17% |
Student > Master | 8 | 13% |
Researcher | 7 | 11% |
Professor > Associate Professor | 4 | 6% |
Student > Bachelor | 4 | 6% |
Other | 11 | 17% |
Unknown | 19 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 19 | 30% |
Biochemistry, Genetics and Molecular Biology | 10 | 16% |
Environmental Science | 3 | 5% |
Materials Science | 2 | 3% |
Medicine and Dentistry | 2 | 3% |
Other | 9 | 14% |
Unknown | 19 | 30% |