| Title |
Systematic Spatial Bias in DNA Microarray Hybridization Is Caused by Probe Spot Position-Dependent Variability in Lateral Diffusion
|
|---|---|
| Published in |
PLOS ONE, August 2011
|
| DOI | 10.1371/journal.pone.0023727 |
| Pubmed ID | |
| Authors |
Doris Steger, David Berry, Susanne Haider, Matthias Horn, Michael Wagner, Roman Stocker, Alexander Loy |
| Abstract |
The hybridization of nucleic acid targets with surface-immobilized probes is a widely used assay for the parallel detection of multiple targets in medical and biological research. Despite its widespread application, DNA microarray technology still suffers from several biases and lack of reproducibility, stemming in part from an incomplete understanding of the processes governing surface hybridization. In particular, non-random spatial variations within individual microarray hybridizations are often observed, but the mechanisms underpinning this positional bias remain incompletely explained. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Czechia | 1 | 2% |
| Germany | 1 | 2% |
| Canada | 1 | 2% |
| Unknown | 42 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 30% |
| Student > Ph. D. Student | 12 | 26% |
| Student > Master | 7 | 15% |
| Student > Bachelor | 3 | 7% |
| Lecturer | 2 | 4% |
| Other | 2 | 4% |
| Unknown | 6 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 33% |
| Biochemistry, Genetics and Molecular Biology | 7 | 15% |
| Engineering | 4 | 9% |
| Environmental Science | 3 | 7% |
| Physics and Astronomy | 2 | 4% |
| Other | 7 | 15% |
| Unknown | 8 | 17% |