Title |
MicroRNA-22 Regulates Hypoxia Signaling in Colon Cancer Cells
|
---|---|
Published in |
PLOS ONE, May 2011
|
DOI | 10.1371/journal.pone.0020291 |
Pubmed ID | |
Authors |
Munekazu Yamakuchi, Shusuke Yagi, Takashi Ito, Charles J. Lowenstein |
Abstract |
MicroRNAs (MiRNAs) are short, non-coding RNA that regulate a variety of cellular functions by suppressing target protein expression. We hypothesized that a set of microRNA regulate tumor responses to hypoxia by inhibiting components of the hypoxia signaling pathway. We found that miR-22 expression in human colon cancer is lower than in normal colon tissue. We also found that miR-22 controls hypoxia inducible factor 1α (HIF-1α) expression in the HCT116 colon cancer cell line. Over-expression of miR-22 inhibits HIF-1α expression, repressing vascular endothelial growth factor (VEGF) production during hypoxia. Conversely, knockdown of endogenous miR-22 enhances hypoxia induced expression of HIF-1α and VEGF. The conditioned media from cells over-expressing miR-22 contain less VEGF protein than control cells, and also induce less endothelial cell growth and invasion, suggesting miR-22 in adjacent cells influences endothelial cell function. Taken together, our data suggest that miR-22 might have an anti-angiogenic effect in colon cancer. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 1% |
France | 1 | 1% |
Ireland | 1 | 1% |
Brazil | 1 | 1% |
India | 1 | 1% |
United Kingdom | 1 | 1% |
Denmark | 1 | 1% |
Japan | 1 | 1% |
United States | 1 | 1% |
Other | 1 | 1% |
Unknown | 73 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 20 | 24% |
Researcher | 19 | 23% |
Student > Master | 14 | 17% |
Student > Bachelor | 4 | 5% |
Other | 4 | 5% |
Other | 15 | 18% |
Unknown | 7 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 36 | 43% |
Biochemistry, Genetics and Molecular Biology | 16 | 19% |
Medicine and Dentistry | 15 | 18% |
Immunology and Microbiology | 2 | 2% |
Nursing and Health Professions | 1 | 1% |
Other | 3 | 4% |
Unknown | 10 | 12% |