Title |
Endocannabinoids Generated by Ca2+ or by Metabotropic Glutamate Receptors Appear to Arise from Different Pools of Diacylglycerol Lipase
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Published in |
PLOS ONE, January 2011
|
DOI | 10.1371/journal.pone.0016305 |
Pubmed ID | |
Authors |
Longhua Zhang, Meina Wang, Tiziana Bisogno, Vincenzo Di Marzo, Bradley E. Alger |
Abstract |
The identity and subcellular sources of endocannabinoids (eCBs) will shape their ability to affect synaptic transmission and, ultimately, behavior. Recent discoveries support the conclusion that 2-arachidonoyl glycerol, 2-AG, is the major signaling eCB, however, some important issues remain open. 2-AG can be synthesized by a mechanism that is strictly Ca(2+)-dependent, and another that is initiated by G-protein coupled receptors (GPCRs) and facilitated by Ca(2+). An important question is whether or not the 2-AG in these cases is synthesized by the same pool of diacylglycerol lipase alpha (DAGLα). Using whole-cell voltage-clamp techniques in CA1 pyramidal cells in acute in vitro rat hippocampal slices, we investigated two mechanistically distinct eCB-mediated responses to address this issue. We now report that pharmacological inhibitors of DGLα have quantitatively different effects on eCB-mediated responses triggered by different stimuli, suggesting that functional, and perhaps physical, distinctions among pools of DAGLα exist. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 3% |
Canada | 2 | 3% |
Germany | 1 | 2% |
Unknown | 55 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 14 | 23% |
Student > Ph. D. Student | 10 | 17% |
Student > Master | 8 | 13% |
Student > Bachelor | 7 | 12% |
Professor | 6 | 10% |
Other | 10 | 17% |
Unknown | 5 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 26 | 43% |
Neuroscience | 7 | 12% |
Chemistry | 5 | 8% |
Medicine and Dentistry | 5 | 8% |
Psychology | 2 | 3% |
Other | 7 | 12% |
Unknown | 8 | 13% |