| Title |
Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
|
|---|---|
| Published in |
PLOS ONE, January 2011
|
| DOI | 10.1371/journal.pone.0015926 |
| Pubmed ID | |
| Authors |
Tim J. Schuijt, Sukanya Narasimhan, Sirlei Daffre, Kathleen DePonte, Joppe W. R. Hovius, Cornelis van't Veer, Tom van der Poll, Kamran Bakhtiari, Joost C. M. Meijers, Eric T. Boder, Alje P. van Dam, Erol Fikrig |
| Abstract |
Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary antigens that react with tick-immune serum. We constructed an Ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identified five salivary antigens. Four of these proteins, designated P8, P19, P23 and P32, had a predicted signal sequence. We generated recombinant (r) P8, P19 and P23 in a Drosophila expression system for functional and immunization studies. rP8 showed anti-complement activity and rP23 demonstrated anti-coagulant activity. Ixodes scapularis feeding was significantly impaired when nymphs were fed on rabbits immunized with a cocktail of rP8, rP19 and rP23, a hall mark of tick-immunity. These studies also suggest that these antigens may serve as potential vaccine candidates to thwart tick feeding. |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 1% |
| United States | 1 | 1% |
| Turkey | 1 | 1% |
| Unknown | 71 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 24% |
| Researcher | 11 | 15% |
| Professor | 9 | 12% |
| Student > Doctoral Student | 7 | 9% |
| Student > Master | 7 | 9% |
| Other | 10 | 14% |
| Unknown | 12 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 31% |
| Biochemistry, Genetics and Molecular Biology | 12 | 16% |
| Medicine and Dentistry | 10 | 14% |
| Veterinary Science and Veterinary Medicine | 5 | 7% |
| Nursing and Health Professions | 2 | 3% |
| Other | 7 | 9% |
| Unknown | 15 | 20% |