| Title |
A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy
|
|---|---|
| Published in |
PLOS ONE, December 2010
|
| DOI | 10.1371/journal.pone.0015732 |
| Pubmed ID | |
| Authors |
Ken Coppieters, Marianne M. Martinic, William B. Kiosses, Natalie Amirian, Matthias von Herrath |
| Abstract |
Type 1 diabetes (T1D) is characterized by the immune-mediated destruction of beta cells in the pancreas. Little is known about the in vivo dynamic interactions between T cells and beta cells or the kinetic behavior of other immune cell subsets in the pancreatic islets. Utilizing multiphoton microscopy we have designed a technique that allows for the real-time visualization of diabetogenic T cells and dendritic cells in pancreatic islets in a live animal, including their interplay with beta cells and the vasculature. Using a custom designed stage, the pancreas was surgically exposed under live conditions so that imaging of islets under intact blood pressure and oxygen supply became possible. We demonstrate here that this approach allows for the tracking of diabetogenic leukocytes as well as vascularization phenotype of islets and accumulation of dendritic cells in islets during diabetes pathogenesis. This technique should be useful in mapping crucial kinetic events in T1D pathogenesis and in testing the impact of immune based interventions on T cell migration, extravasation and islet destruction. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 2 | 3% |
| Germany | 1 | 2% |
| Unknown | 60 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 24% |
| Researcher | 15 | 24% |
| Professor > Associate Professor | 5 | 8% |
| Student > Doctoral Student | 4 | 6% |
| Professor | 4 | 6% |
| Other | 13 | 21% |
| Unknown | 7 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 29% |
| Immunology and Microbiology | 11 | 17% |
| Medicine and Dentistry | 8 | 13% |
| Physics and Astronomy | 5 | 8% |
| Nursing and Health Professions | 3 | 5% |
| Other | 9 | 14% |
| Unknown | 9 | 14% |