Title |
Identification of Novel Inhibitors of Dietary Lipid Absorption Using Zebrafish
|
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Published in |
PLOS ONE, August 2010
|
DOI | 10.1371/journal.pone.0012386 |
Pubmed ID | |
Authors |
Justin D. Clifton, Edinson Lucumi, Michael C. Myers, Andrew Napper, Kotaro Hama, Steven A. Farber, Amos B. Smith, Donna M. Huryn, Scott L. Diamond, Michael Pack |
Abstract |
Pharmacological inhibition of dietary lipid absorption induces favorable changes in serum lipoprotein levels in patients that are at risk for cardiovascular disease and is considered an adjuvant or alternative treatment with HMG-CoA reductase inhibitors (statins). Here we demonstrate the feasibility of identifying novel inhibitors of intestinal lipid absorption using the zebrafish system. A pilot screen of an unbiased chemical library identified novel compounds that inhibited processing of fluorescent lipid analogues in live zebrafish larvae. Secondary assays identified those compounds suitable for testing in mammals and provided insight into mechanism of action, which for several compounds could be distinguished from ezetimibe, a drug used to inhibit cholesterol absorption in humans that broadly inhibited lipid absorption in zebrafish larvae. These findings support the utility of zebrafish screening assays to identify novel compounds that target complex physiological processes. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 1% |
United States | 1 | 1% |
Unknown | 77 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 21 | 27% |
Student > Ph. D. Student | 16 | 20% |
Professor > Associate Professor | 8 | 10% |
Student > Master | 8 | 10% |
Student > Bachelor | 7 | 9% |
Other | 10 | 13% |
Unknown | 9 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 31 | 39% |
Biochemistry, Genetics and Molecular Biology | 11 | 14% |
Medicine and Dentistry | 9 | 11% |
Environmental Science | 3 | 4% |
Chemistry | 3 | 4% |
Other | 11 | 14% |
Unknown | 11 | 14% |