| Title |
Apparent Non-Canonical Trans-Splicing Is Generated by Reverse Transcriptase In Vitro
|
|---|---|
| Published in |
PLOS ONE, August 2010
|
| DOI | 10.1371/journal.pone.0012271 |
| Pubmed ID | |
| Authors |
Jonathan Houseley, David Tollervey |
| Abstract |
Trans-splicing, the in vivo joining of two independently transcribed RNA molecules, is well characterized in lower eukaryotes, but was long thought absent from metazoans. However, recent bioinformatic analyses of EST sequences suggested widespread trans-splicing in mammals. These apparently spliced transcripts generally lacked canonical splice sites, leading us to question their authenticity. Particularly, the native ability of reverse transcriptase enzymes to template switch during transcription could produce apparently trans-spliced sequences. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 165 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 2% |
| United Kingdom | 2 | 1% |
| Germany | 1 | <1% |
| Canada | 1 | <1% |
| Australia | 1 | <1% |
| Spain | 1 | <1% |
| Poland | 1 | <1% |
| Unknown | 154 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 49 | 30% |
| Researcher | 41 | 25% |
| Student > Master | 15 | 9% |
| Student > Doctoral Student | 9 | 5% |
| Student > Bachelor | 9 | 5% |
| Other | 27 | 16% |
| Unknown | 15 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 78 | 47% |
| Biochemistry, Genetics and Molecular Biology | 43 | 26% |
| Computer Science | 8 | 5% |
| Medicine and Dentistry | 5 | 3% |
| Environmental Science | 3 | 2% |
| Other | 12 | 7% |
| Unknown | 16 | 10% |