| Title |
Real-Time Relative qPCR without Reference to Control Samples and Estimation of Run-Specific PCR Parameters from Run-Internal Mini-Standard Curves
|
|---|---|
| Published in |
PLOS ONE, July 2010
|
| DOI | 10.1371/journal.pone.0011723 |
| Pubmed ID | |
| Authors |
Jens Magnus Bernth Jensen, Mikkel Steen Petersen, Marc Stegger, Lars J. Østergaard, Bjarne K. Møller |
| Abstract |
Real-Time quantitative PCR is an important tool in research and clinical settings. Here, we describe two new approaches that broaden the scope of real-time quantitative PCR; namely, run-internal mini standard curves (RIMS) and direct real-time relative quantitative PCR (drqPCR). RIMS are an efficient alternative to traditional standard curves and provide both run-specific and target-specific estimates of PCR parameters. The drqPCR enables direct estimation of target ratios without reference to conventional control samples. |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 2% |
| Germany | 1 | 2% |
| France | 1 | 2% |
| Australia | 1 | 2% |
| Brazil | 1 | 2% |
| United States | 1 | 2% |
| Unknown | 46 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 21 | 40% |
| Student > Ph. D. Student | 8 | 15% |
| Professor > Associate Professor | 4 | 8% |
| Student > Master | 4 | 8% |
| Professor | 3 | 6% |
| Other | 5 | 10% |
| Unknown | 7 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 46% |
| Medicine and Dentistry | 7 | 13% |
| Biochemistry, Genetics and Molecular Biology | 4 | 8% |
| Environmental Science | 2 | 4% |
| Immunology and Microbiology | 2 | 4% |
| Other | 7 | 13% |
| Unknown | 6 | 12% |