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Cost-Effective Sequencing of Full-Length cDNA Clones Powered by a De Novo-Reference Hybrid Assembly

Overview of attention for article published in PLOS ONE, May 2010
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Title
Cost-Effective Sequencing of Full-Length cDNA Clones Powered by a De Novo-Reference Hybrid Assembly
Published in
PLOS ONE, May 2010
DOI 10.1371/journal.pone.0010517
Pubmed ID
Authors

Reginaldo M. Kuroshu, Junichi Watanabe, Sumio Sugano, Shinichi Morishita, Yutaka Suzuki, Masahiro Kasahara

Abstract

Sequencing full-length cDNA clones is important to determine gene structures including alternative splice forms, and provides valuable resources for experimental analyses to reveal the biological functions of coded proteins. However, previous approaches for sequencing cDNA clones were expensive or time-consuming, and therefore, a fast and efficient sequencing approach was demanded.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Italy 1 2%
United Kingdom 1 2%
Canada 1 2%
China 1 2%
United States 1 2%
Unknown 48 89%

Demographic breakdown

Readers by professional status Count As %
Researcher 18 33%
Student > Ph. D. Student 12 22%
Professor 6 11%
Student > Master 4 7%
Professor > Associate Professor 3 6%
Other 10 19%
Unknown 1 2%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 56%
Biochemistry, Genetics and Molecular Biology 9 17%
Computer Science 5 9%
Medicine and Dentistry 4 7%
Unknown 6 11%