| Title |
Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples
|
|---|---|
| Published in |
PLOS ONE, April 2010
|
| DOI | 10.1371/journal.pone.0010391 |
| Pubmed ID | |
| Authors |
Ulrich Leischner, Anja Schierloh, Walter Zieglgänsberger, Hans-Ulrich Dodt |
| Abstract |
Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three-dimensional observation of cellular structures in various tissue types without complicated staining techniques. |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 2% |
| United States | 1 | 2% |
| Serbia | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 53 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 32% |
| Researcher | 15 | 26% |
| Student > Master | 6 | 11% |
| Other | 3 | 5% |
| Professor > Associate Professor | 3 | 5% |
| Other | 8 | 14% |
| Unknown | 4 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Physics and Astronomy | 12 | 21% |
| Agricultural and Biological Sciences | 10 | 18% |
| Engineering | 8 | 14% |
| Medicine and Dentistry | 7 | 12% |
| Neuroscience | 4 | 7% |
| Other | 8 | 14% |
| Unknown | 8 | 14% |