| Title |
Full Genome Characterisation of Bluetongue Virus Serotype 6 from the Netherlands 2008 and Comparison to Other Field and Vaccine Strains
|
|---|---|
| Published in |
PLOS ONE, April 2010
|
| DOI | 10.1371/journal.pone.0010323 |
| Pubmed ID | |
| Authors |
Sushila Maan, Narender S. Maan, Piet A. van Rijn, René G. P. van Gennip, Anna Sanders, Isabel M. Wright, Carrie Batten, Bernd Hoffmann, Michael Eschbaumer, Chris A. L. Oura, Abraham C. Potgieter, Kyriaki Nomikou, Peter P.C. Mertens |
| Abstract |
In mid September 2008, clinical signs of bluetongue (particularly coronitis) were observed in cows on three different farms in eastern Netherlands (Luttenberg, Heeten, and Barchem), two of which had been vaccinated with an inactivated BTV-8 vaccine (during May-June 2008). Bluetongue virus (BTV) infection was also detected on a fourth farm (Oldenzaal) in the same area while testing for export. BTV RNA was subsequently identified by real time RT-PCR targeting genome-segment (Seg-) 10, in blood samples from each farm. The virus was isolated from the Heeten sample (IAH "dsRNA virus reference collection" [dsRNA-VRC] isolate number NET2008/05) and typed as BTV-6 by RT-PCR targeting Seg-2. Sequencing confirmed the virus type, showing an identical Seg-2 sequence to that of the South African BTV-6 live-vaccine-strain. Although most of the other genome segments also showed very high levels of identity to the BTV-6 vaccine (99.7 to 100%), Seg-10 showed greatest identity (98.4%) to the BTV-2 vaccine (RSAvvv2/02), indicating that NET2008/05 had acquired a different Seg-10 by reassortment. Although Seg-7 from NET2008/05 was also most closely related to the BTV-6 vaccine (99.7/100% nt/aa identity), the Seg-7 sequence derived from the blood sample of the same animal (NET2008/06) was identical to that of the Netherlands BTV-8 (NET2006/04 and NET2007/01). This indicates that the blood contained two different Seg-7 sequences, one of which (from the BTV-6 vaccine) was selected during virus isolation in cell-culture. The predominance of the BTV-8 Seg-7 in the blood sample suggests that the virus was in the process of reassorting with the northern field strain of BTV-8. Two genome segments of the virus showed significant differences from the BTV-6 vaccine, indicating that they had been acquired by reassortment event with BTV-8, and another unknown parental-strain. However, the route by which BTV-6 and BTV-8 entered northern Europe was not established. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Ukraine | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 80 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 1% |
| South Africa | 1 | 1% |
| United Kingdom | 1 | 1% |
| Argentina | 1 | 1% |
| Spain | 1 | 1% |
| Unknown | 75 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 21% |
| Student > Ph. D. Student | 15 | 19% |
| Student > Master | 9 | 11% |
| Student > Bachelor | 6 | 8% |
| Other | 5 | 6% |
| Other | 15 | 19% |
| Unknown | 13 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 28 | 35% |
| Veterinary Science and Veterinary Medicine | 15 | 19% |
| Immunology and Microbiology | 6 | 8% |
| Biochemistry, Genetics and Molecular Biology | 5 | 6% |
| Medicine and Dentistry | 3 | 4% |
| Other | 5 | 6% |
| Unknown | 18 | 23% |