| Title |
Engineering Antigen-Specific T Cells from Genetically Modified Human Hematopoietic Stem Cells in Immunodeficient Mice
|
|---|---|
| Published in |
PLOS ONE, December 2009
|
| DOI | 10.1371/journal.pone.0008208 |
| Pubmed ID | |
| Authors |
Scott G. Kitchen, Michael Bennett, Zoran Galić, Joanne Kim, Qing Xu, Alan Young, Alexis Lieberman, Aviva Joseph, Harris Goldstein, Hwee Ng, Otto Yang, Jerome A. Zack |
| Abstract |
There is a desperate need for effective therapies to fight chronic viral infections. The immune response is normally fastidious at controlling the majority of viral infections and a therapeutic strategy aimed at reestablishing immune control represents a potentially powerful approach towards treating persistent viral infections. We examined the potential of genetically programming human hematopoietic stem cells to generate mature CD8+ cytotoxic T lymphocytes that express a molecularly cloned, "transgenic" human anti-HIV T cell receptor (TCR). Anti-HIV TCR transduction of human hematopoietic stem cells directed the maturation of a large population of polyfunctional, HIV-specific CD8+ cells capable of recognizing and killing viral antigen-presenting cells. Thus, through this proof-of-concept we propose that genetic engineering of human hematopoietic stem cells will allow the tailoring of effector T cell responses to fight HIV infection or other diseases that are characterized by the loss of immune control. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 2% |
| United States | 1 | 2% |
| Germany | 1 | 2% |
| South Africa | 1 | 2% |
| Unknown | 53 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 30% |
| Researcher | 12 | 21% |
| Student > Master | 8 | 14% |
| Student > Bachelor | 6 | 11% |
| Professor > Associate Professor | 4 | 7% |
| Other | 7 | 12% |
| Unknown | 3 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 39% |
| Medicine and Dentistry | 11 | 19% |
| Biochemistry, Genetics and Molecular Biology | 8 | 14% |
| Immunology and Microbiology | 6 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Other | 5 | 9% |
| Unknown | 3 | 5% |