| Title |
Whole Genome Amplification and De novo Assembly of Single Bacterial Cells
|
|---|---|
| Published in |
PLOS ONE, September 2009
|
| DOI | 10.1371/journal.pone.0006864 |
| Pubmed ID | |
| Authors |
Sébastien Rodrigue, Rex R. Malmstrom, Aaron M. Berlin, Bruce W. Birren, Matthew R. Henn, Sallie W. Chisholm |
| Abstract |
Single-cell genome sequencing has the potential to allow the in-depth exploration of the vast genetic diversity found in uncultured microbes. We used the marine cyanobacterium Prochlorococcus as a model system for addressing important challenges facing high-throughput whole genome amplification (WGA) and complete genome sequencing of individual cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 487 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 17 | 3% |
| Spain | 6 | 1% |
| Germany | 4 | <1% |
| Brazil | 4 | <1% |
| Norway | 3 | <1% |
| Canada | 3 | <1% |
| United Kingdom | 3 | <1% |
| India | 3 | <1% |
| Denmark | 3 | <1% |
| Other | 14 | 3% |
| Unknown | 427 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 144 | 30% |
| Student > Ph. D. Student | 116 | 24% |
| Student > Master | 47 | 10% |
| Student > Bachelor | 33 | 7% |
| Professor > Associate Professor | 28 | 6% |
| Other | 82 | 17% |
| Unknown | 37 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 279 | 57% |
| Biochemistry, Genetics and Molecular Biology | 73 | 15% |
| Environmental Science | 27 | 6% |
| Immunology and Microbiology | 13 | 3% |
| Computer Science | 11 | 2% |
| Other | 41 | 8% |
| Unknown | 43 | 9% |