Title |
Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
|
---|---|
Published in |
PLOS ONE, July 2009
|
DOI | 10.1371/journal.pone.0006441 |
Pubmed ID | |
Authors |
Jiayuan Quan, Jingdong Tian |
Abstract |
High-throughput genomics and the emerging field of synthetic biology demand ever more convenient, economical, and efficient technologies to assemble and clone genes, gene libraries and synthetic pathways. Here, we describe the development of a novel and extremely simple cloning method, circular polymerase extension cloning (CPEC). This method uses a single polymerase to assemble and clone multiple inserts with any vector in a one-step reaction in vitro. No restriction digestion, ligation, or single-stranded homologous recombination is required. In this study, we elucidate the CPEC reaction mechanism and demonstrate its usage in demanding synthetic biology applications such as one-step assembly and cloning of complex combinatorial libraries and multi-component pathways. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 60% |
Netherlands | 1 | 20% |
Unknown | 1 | 20% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 3 | 60% |
Members of the public | 2 | 40% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 18 | 2% |
United Kingdom | 11 | 1% |
Germany | 6 | <1% |
Denmark | 3 | <1% |
Belgium | 3 | <1% |
Uruguay | 3 | <1% |
Austria | 2 | <1% |
Australia | 2 | <1% |
Netherlands | 2 | <1% |
Other | 11 | 1% |
Unknown | 885 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 213 | 23% |
Student > Master | 165 | 17% |
Researcher | 163 | 17% |
Student > Bachelor | 127 | 13% |
Student > Doctoral Student | 38 | 4% |
Other | 113 | 12% |
Unknown | 127 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 415 | 44% |
Biochemistry, Genetics and Molecular Biology | 243 | 26% |
Engineering | 35 | 4% |
Chemistry | 26 | 3% |
Immunology and Microbiology | 18 | 2% |
Other | 64 | 7% |
Unknown | 145 | 15% |