| Title |
Mapping Accuracy of Short Reads from Massively Parallel Sequencing and the Implications for Quantitative Expression Profiling
|
|---|---|
| Published in |
PLOS ONE, July 2009
|
| DOI | 10.1371/journal.pone.0006323 |
| Pubmed ID | |
| Authors |
Nicola Palmieri, Christian Schlötterer |
| Abstract |
Massively parallel sequencing offers an enormous potential for expression profiling, in particular for interspecific comparisons. Currently, different platforms for massively parallel sequencing are available, which differ in read length and sequencing costs. The 454-technology offers the highest read length. The other sequencing technologies are more cost effective, on the expense of shorter reads. Reliable expression profiling by massively parallel sequencing depends crucially on the accuracy to which the reads could be mapped to the corresponding genes. |
Mendeley readers
The data shown below were compiled from readership statistics for 170 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 9 | 5% |
| Italy | 3 | 2% |
| China | 2 | 1% |
| Norway | 2 | 1% |
| Netherlands | 1 | <1% |
| France | 1 | <1% |
| Brazil | 1 | <1% |
| Sweden | 1 | <1% |
| Germany | 1 | <1% |
| Other | 8 | 5% |
| Unknown | 141 | 83% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 62 | 36% |
| Student > Ph. D. Student | 27 | 16% |
| Professor > Associate Professor | 18 | 11% |
| Student > Master | 15 | 9% |
| Other | 11 | 6% |
| Other | 28 | 16% |
| Unknown | 9 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 120 | 71% |
| Biochemistry, Genetics and Molecular Biology | 23 | 14% |
| Computer Science | 5 | 3% |
| Medicine and Dentistry | 5 | 3% |
| Immunology and Microbiology | 2 | 1% |
| Other | 4 | 2% |
| Unknown | 11 | 6% |